Journal: Global Change Biology
Article Title: Nitrogen Redox Controls on Greenhouse Gas Production in Yedoma Taliks
doi: 10.1111/gcb.70356
Figure Lengend Snippet: Methane dynamics in upland yedoma, suggested mechanisms. During summer (left panel), CH4 is oxidized by both anaerobic (at deeper) and aerobic (at shallower layer) methanotrophs (i.e., ANME2d, NC10, and denitrifiers performing N‐AOM). Methane oxidation is coupled to the nitrogen cycle, with N‐AOM linking CH4 and nitrogen transformations. N 2 O is produced and present up to 1 m depth. In winter, near‐freezing temperatures at the soil surface limit microbial activity and methanotrophy. The diminished CH4 oxidation and nitrogen‐related processes at deeper horizons may be related to lower temperatures, functional constraints, or nitrogen availability, leading to decreased N‐AOM, higher CH4 emissions to the atmosphere, and lower N 2 O production. The suggested mechanisms are based on chemical and qPCR profiles, and 16S amplicon‐based sequencing analyses. The background colors represent different soil oxygenation conditions (see Materials and Methods and Data ) (Walter Anthony et al. ).
Article Snippet: qPCR was performed using a CFX Duet qPCR instrument (Bio‐Rad, USA) and analyzed with the CFX Maestro software.
Techniques: Produced, Activity Assay, Functional Assay, Amplification, Sequencing